کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6136054 | 1593645 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Human transcriptome analysis reveals a potential role for active transport in the metabolism of Pseudomonas aeruginosa autoinducers
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
The opportunistic pathogen Pseudomonas aeruginosa employs acyl homoserine lactones (AHL) as signaling compounds to regulate virulence gene expression via quorum sensing. The AHL N-3-oxo-dodecanoyl-l-homoserine lactone (3OC12-HSL) also induces mammalian cell responses, including apoptosis and immune modulation. In certain cell types the apoptotic effects of 3OC12-HSL are mediated via a calcium-dependent signaling pathway, while some pro-inflammatory effects involve intracellular transcriptional regulators. However, the mechanisms by which mammalian cells perceive and respond to 3OC12-HSL are still not completely understood. Here we used microarray analysis to investigate the transcriptional response of human lung epithelial cells after exposure to 3OC12-HSL. These data revealed that mRNA levels for several genes involved in xenobiotic sensing and drug transport were increased in cells exposed to 3OC12-HSL, which led us to examine the intracellular fate of 3OC12-HSL. Using radiolabeled autoinducer uptake assays, we discovered that intracellular 3OC12-HSL levels increased after exposure and achieved maximal levels after 20-30Â min. Intracellular 3OC12-HSL decreased to background levels over the next 90Â min and this process was blocked by pre-treatment with an inhibitor of the ABC transporter ABCA1. Taken together, these data suggest that mammalian cells detect 3OC12-HSL and activate protective mechanisms to expel it from the cell.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 12, Issues 12â13, November 2010, Pages 1042-1050
Journal: Microbes and Infection - Volume 12, Issues 12â13, November 2010, Pages 1042-1050
نویسندگان
Amanda Bryan, Chase Watters, Lars Koenig, Eunseog Youn, Aaron Olmos, Guigen Li, Simon C. Williams, Kendra P. Rumbaugh,