کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6136438 1225147 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative proteomic analysis of a Haemophilus parasuis SC096 mutant deficient in the outer membrane protein P5
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Comparative proteomic analysis of a Haemophilus parasuis SC096 mutant deficient in the outer membrane protein P5
چکیده انگلیسی

Outer membrane protein A (OmpA) is a major structural component of the outer membranes and functions as a multifaceted molecular with many diverse roles in Gram-negative bacteria. In Haemophilus parasuis, OmpA has been recognized and named as OmpP5 in genomic literature. In this study, to determine the precise functions of OmpP5, an ompP5 deficient mutant (ΔompP5) of a H. parasuis serovar 4 filed strain SC096 was constructed using a natural transformation method. Compared to the wild-type SC096 strain, the ΔompP5 mutant displayed a detectable delay in growth. However, the wild-type and mutant strains were indistinguishable with respect to the other phenotypes including resistance to killing by porcine and rabbit sera, adhesion to and invasion of porcine umbilicus veins endothelial cells (PUVEC) and porcine kidney epithelial cells (PK-15). To analyze the differences of proteome expression between wild-type and mutant strains, a 2-dimensional gel electrophoresis (2-DE)-based proteomics comparison was performed. There were 24 differentially expressed proteins which were mainly involved in carbohydrate, lipid, nucleotide and amino acid metabolism, or served as transcription and translation factors and chaperone proteins. Collectively, loss of OmpP5 expression in the H. parasuis SC096 strain resulted in global protein expression changes which might be responsible for novel phenotypes occurred in ΔompP5 mutant.

► We first constructed a ΔompP5 mutant of the H. parasuis SC096 strain. ► The ΔompP5 mutant displayed a detectable delay in growth, but it still retained the ability to resist to the serum bactericidal activity, adhere to and invade host cells. ► Loss of OmpP5 expression resulted in global protein expression changes which might be responsible for novel phenotypes occurred in ΔompP5 mutant.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 52, Issue 2, February 2012, Pages 117-124
نویسندگان
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