کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6138701 | 1594221 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Complement factors alter the amount of PrPSc in primary-cultured mouse cortical neurons associated with increased membrane permeability
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We examined the effects of complement factors on primary-cultured neurons infected with prions. The amount of protease K (PK)-resistant abnormal form of prion protein (PrPSc) reached a maximum level at 12 and 16 days post exposure (dpe) in 22L- and Chandler-infected neurons, respectively. In Chandler-infected neurons, the reaction of complement factors C1q, C3 and C9 significantly increased membrane permeability. This was followed by a decrease of PK-resistant PrPSc at 16 and 20Â dpe. In contrast, in 22L-infected neurons, the effects of complement factors were observed at 12 and 16Â dpe, but not at 20Â dpe. Membrane permeability also increased in 22L-infected neurons by reaction of complement factor C3, but interestingly, the amount of PK-resistant PrPSc initially decreased, and then increased. These results suggest that the reactivity of complement factors in prion-infected neurons depends on the amount of PrPSc and the prion strain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 496, September 2016, Pages 9-20
Journal: Virology - Volume 496, September 2016, Pages 9-20
نویسندگان
Rie Hasebe, Misaki Tanaka, Akio Suzuki, Takeshi Yamasaki, Motohiro Horiuchi,