کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6139497 1594238 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of the complete genome of Barley yellow striate mosaic virus reveals a nested gene encoding a small hydrophobic protein
ترجمه فارسی عنوان
مشخصه کامل ژنوم ویروس موزاییک زرد جو، نشان دهنده یک ژن تویستی است که پروتئین هیدروفوب کوچک را رمزگذاری می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- The complete genome of Barley yellow striate mosaic virus is obtained.
- A novel nested gene is found in the genome of BYSMV.
- The nested gene is translated from the mRNA via a leaky scanning mechanism.
- The nested gene encodes a small hydrophobic protein.
- The siRNAs derived from BYSMV is cloned and analyzed.

Barley yellow striate mosaic virus (BYSMV), a member of the genus Cytorhabdovirus, causes serious crop losses in agriculture. Here, we have cloned the BYSMV-derived small interfering RNAs (siRNAs), assembled the siRNAs and used RT-PCR to reconstruct the BYSMV genome. The genome consists of 12,706 nucleotides and encodes ten predicted genes from the antigenomic strand. The major BYSMV structural proteins share identities ranging from 35% to 62% with northern cereal mosaic virus (NCMV) counterparts. A notable difference is that BYSMV contains three transcriptional units residing between the P and M genes compared with four units in the corresponding region of NCMV. Unexpectedly, the middle mRNA in this region encodes gene5 nested in an alternative frame within gene4 via a leaky scanning mechanism. The gene5 encodes a small hydrophobic protein targeting to the endoplasmic reticulum (ER). To our knowledge, this is the first report of nested gene in plant rhabdoviruses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 478, April 2015, Pages 112-122
نویسندگان
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