کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6140984 | 1227190 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates
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کلمات کلیدی
ORFTMDffuHCVccHCVppfocus forming units - تمرکز واحدهای تشکیل شده استNeutralization - خنثی سازیTrans-membrane domain - دامنه غشاییopen reading frame - قاب خواندن بازCell culture model - مدل کشت سلولیUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهHepatitis C virus - هپاتیت سیGenotype - ژنوتیپ
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates Construction and characterization of infectious hepatitis C virus chimera containing structural proteins directly from genotype 1b clinical isolates](/preview/png/6140984.png)
چکیده انگلیسی
HCV genotype is a major determinant of clinical outcome, and GT1b HCV infection is the most difficult to treat and also the predominant genotype in East Asia and Europe. We developed 1b/JFH-1 inter-genotypic recombinants containing the structural genes (Core, E1, E2), p7 and the 1stTMD of NS2 directly from GT1b clinical isolates. Through a cloning selection strategy, we obtained 4 functional clones from 3 cases of GT1b patients' sera, which could produce infectious viruses in Huh7.5.1 cells. Sequencing analysis of recovered viruses from serial passage and reverse genetics revealed that adaptive mutations in the GT1b-originated region were enough for the enhancement of infectivity. A monoclonal antibody to E2 and original patient sera could efficiently block 3 of the viruses (26C3mt, 52B6mt and 79L9) while had little effect on 26C6mt viruses. The availability of 1b/JFH-1 chimeric viruses will be important for studies of isolate-specific neutralization and useful in evaluating antiviral therapies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 443, Issue 1, 15 August 2013, Pages 80-88
Journal: Virology - Volume 443, Issue 1, 15 August 2013, Pages 80-88
نویسندگان
Jie Lu, Wanyin Tao, Rui Li, Yu Xiang, Nan Zhang, Xiaogang Xiang, Qing Xie, Jin Zhong,