Human papillomavirus type 16 E7 oncoprotein engages but does not abrogate the mitotic spindle assembly checkpoint

► We examined the effect of HPV16 E7 on mitotic checkpoint activity. ► E7 inhibited cyclin B degradation. ► Multiple E7 domains contribute to this activity. ► Inhibition of cyclin B degradation reflects spindle assembly checkpoint engagement. ► Spindle assembly checkpoint activation in response to microtubule poisons is normal.