کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6141294 | 1227232 | 2012 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of the newly identified human parvovirus 4 in the family Parvoviridae
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular characterization of the newly identified human parvovirus 4 in the family Parvoviridae Molecular characterization of the newly identified human parvovirus 4 in the family Parvoviridae](/preview/png/6141294.png)
چکیده انگلیسی
Human parvovirus 4 (PARV4) is an emerging human virus, and little is known about the molecular aspects of PARV4 apart from its incomplete genome sequence, which lacks information of the termini. We analyzed the gene expression profile of PARV4 using a nearly full-length HPV4 genome in a replication competent system in 293 cells. We found that PARV4 utilizes two promoters to transcribe non-structural protein- and structural protein-encoding mRNAs, respectively, which were polyadenylated at the right end of the genome. Three major proteins, including the large non-structural protein NS1a, whose mRNA is spliced, and capsid proteins VP1 and VP2, were detected. Additional functional analysis of the NS1a revealed its capability to induce cell cycle arrest at G2/M phase in ex vivo-generated human hematopoietic stem cells. Taken together, our characterization of the molecular features of PARV4 suggests that PARV4 represents a new genus in the family Parvoviridae.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 422, Issue 1, 5 January 2012, Pages 59-69
Journal: Virology - Volume 422, Issue 1, 5 January 2012, Pages 59-69
نویسندگان
Sai Lou, Baoyan Xu, Qinfeng Huang, Ning Zhi, Fang Cheng, Susan Wong, Kevin Brown, Eric Delwart, Zhengwen Liu, Jianming Qiu,