کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6141370 1227244 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of tolerated insertion sites in poliovirus non-structural proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Identification of tolerated insertion sites in poliovirus non-structural proteins
چکیده انگلیسی

Insertion of nucleotide sequences encoding “tags” that can be expressed in specific viral proteins during an infection is a useful strategy for purifying viral proteins and their functional complexes from infected cells and/or for visualizing the dynamics of their subcellular location over time. To identify regions in the poliovirus polyprotein that could potentially accommodate insertion of tags, transposon-mediated insertion mutagenesis was applied to the entire nonstructural protein-coding region of the poliovirus genome, followed by selection of genomes capable of generating infectious, viable viruses. This procedure allowed us to identify at least one site in each viral nonstructural protein, except protein 2C, in which a minimum of five amino acids could be inserted. The distribution of these sites is analyzed from the perspective of their protein structural context and from the perspective of virus evolution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 409, Issue 1, 5 January 2011, Pages 1-11
نویسندگان
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