کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6141883 1227323 2009 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of domains of the Tomato spotted wilt virus NSm protein involved in tubule formation, movement and symptomatology
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Identification of domains of the Tomato spotted wilt virus NSm protein involved in tubule formation, movement and symptomatology
چکیده انگلیسی

Deletion and alanine-substitution mutants of the Tomato spotted wilt virus NSm protein were generated to identify domains involved in tubule formation, movement and symptomatology using a heterologous Tobacco mosaic virus expression system. Two regions of NSm, G19-S159 and G209-V283, were required for both tubule formation in protoplasts and cell-to-cell movement in plants, indicating a correlation between these activities. Three amino acid groups, D154, EYKK205-208 and EEEEE284-288 were linked with long-distance movement in Nicotiana benthamiana. EEEEE284-288 was essential for NSm-mediated long-distance movement, whereas D154 was essential for tubule formation and cell-to-cell movement; indicating separate genetic controls for cell-to-cell and long-distance movement. The region I57-N100 was identified as the determinant of foliar necrosis in Nicotiana benthamiana, and mutagenesis of HH93-94 greatly reduced necrosis. These findings are likely applicable to other tospovirus species, especially those within the 'New World' group as NSm sequences are highly conserved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 390, Issue 1, 20 July 2009, Pages 110-121
نویسندگان
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