کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6141944 | 1227358 | 2008 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phosphorylation of the integrase protein of coliphage HK022
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phosphorylation of the integrase protein of coliphage HK022 Phosphorylation of the integrase protein of coliphage HK022](/preview/png/6141944.png)
چکیده انگلیسی
The integrase (Int) proteins of coliphages HK022 and λ, are phosphorylated in one or more of their tyrosine residues. In Int of HK022 the phosphorylated residue(s) belong to its core-binding/catalytic domains. Wzc, a protein tyrosine kinase of Escherichia coli, is not required for Int phosphorylation in vivo, however, it can transphosphorylate the conserved Tyr342 catalytic residue of Int in vitro. Int purified from cells that overexpress Wzc has a reduced activity in vitro. In vivo, the lysogenization of wild type HK022 as well as of λ is not affected by the overexpression of Wzc. However, the nin5 mutant of λ, which lacks a protein-tyrosine phosphatase gene, shows a significantly reduced lysogenization. It is suggested that phosphorylation of Int by Wzc down regulates the activity of Int.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 375, Issue 2, 5 June 2008, Pages 383-390
Journal: Virology - Volume 375, Issue 2, 5 June 2008, Pages 383-390
نویسندگان
Mikhail Kolot, Rena Gorovits, Nava Silberstein, Boris Fichtman, Ezra Yagil,