کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6142677 | 1228209 | 2013 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High molecular weight complex analysis of Epstein-Barr virus Latent Membrane Protein 1 (LMP-1): Structural insights into LMP-1's homo-oligomerization and lipid raft association
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کلمات کلیدی
N-ethylmaleimideBismaleimidohexaneLMP-1TMEABMHTRAFIFNαEGSBN-PAGEOligomerizationblue native polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید آبی بومیEBV - اپشتین بار ویروسinterferon alpha - اینترفرون آلفاComplex formation - تشکیل پیچیدهLipid raft - قایق های چربNEM - نهEpstein–Barr virus - ویروس Epstein-BarrLatent membrane protein-1 - پروتئین غشایی غلیظ -1
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
LMP-1 is a constitutively active Tumor Necrosis Factor Receptor analog encoded by Epstein-Barr virus. LMP-1 activation correlates with oligomerization and raft localization, but direct evidence of LMP-1 oligomers is limited. We report that LMP-1 forms multiple high molecular weight native LMP-1 complexes when analyzed by BN-PAGE, the largest of which are enriched in detergent resistant membranes. The largest of these high molecular weight complexes are not formed by purified LMP-1 or by loss of function LMP-1 mutants. Consistent with these results we find a dimeric form of LMP-1 that can be stabilized by disulfide crosslinking. We identify cysteine 238 in the C-terminus of LMP-1 as the crosslinked cysteine. Disulfide crosslinking occurs post-lysis but the dimer can be crosslinked in intact cells with membrane permeable crosslinkers. LMP-1/C238A retains wild type LMP-1 NF-κB activity. LMP-1's TRAF binding, raft association and oligomerization are associated with the dimeric form of LMP-1. Our results suggest the possibility that the observed dimeric species results from inter-oligomeric crosslinking of LMP-1 molecules in adjacent core LMP-1 oligomers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 178, Issue 2, 26 December 2013, Pages 314-327
Journal: Virus Research - Volume 178, Issue 2, 26 December 2013, Pages 314-327
نویسندگان
Christopher M. Wrobel, Timothy R. Geiger, Rebecca N. Nix, Aaron M. Robitaille, Sandra Weigand, Alfredo Cervantes, Miguel Gonzalez, Jennifer M. Martin,