کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6143006 1594379 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adaptation of yellow fever virus 17D to Vero cells is associated with mutations in structural and non-structural protein genes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Adaptation of yellow fever virus 17D to Vero cells is associated with mutations in structural and non-structural protein genes
چکیده انگلیسی

Serial passaging of yellow fever virus 17D in Vero cells was employed to derive seed material for a novel inactivated vaccine, XRX-001. Two independent passaging series identified a novel lysine to arginine mutation at amino acid 160 of the envelope protein, a surface-exposed residue in structural domain I. A third passage series resulted in an isoleucine to methionine mutation at residue 113 of the NS4B protein, a central membrane spanning region of the protein which has previously been associated with Vero cell adaptation of other mosquito-borne flaviviruses. These studies confirm that flavivirus adaptation to growth in Vero cells can be mediated by structural or non-structural protein mutations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 176, Issues 1–2, September 2013, Pages 280-284
نویسندگان
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