The C-terminal region of the hepatitis B virus X protein is required for its stimulation of HBV replication in primary mouse hepatocytes

► Cultured primary mouse hepatocytes retain differentiated status and liver functions. ► Cultured primary mouse hepatocytes retain the same cell cycle status as normal cells. ► C-terminus of HBx is needed for its impact on the cell cycle in primary hepatocytes. ► C-terminus of HBx is needed for its impact on HBV replication in primary hepatocytes. ► HBV replication in primary mouse hepatocytes needs HBx-dependent cell cycle control.