Topology and cellular localization of the small hydrophobic protein of avian metapneumovirus

► We show that AMPV-C SH is an integral membrane protein with NinCout orientation located in both the plasma membrane as well as within intracellular compartments. ► Furthermore, we demonstrate that AMPV-C SH protein localizes in the endoplasmic reticulum (ER), Golgi, and cell surface. ► And is transported through ER-Golgi secretory pathway.