کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6143547 1594389 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a linear epitope on the capsid protein of classical swine fever virus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Identification of a linear epitope on the capsid protein of classical swine fever virus
چکیده انگلیسی

The capsid (C) protein of Classical swine fever virus (CSFV) is proposed to play an essential role in the replication and translation of the viral RNA. In this study, a monoclonal antibody (mAb) directed against the C protein was generated with the recombinant C protein expressed in Escherichia coli as immunogen. IFA and IPMA analysis showed that the native C protein of CSFV virions was reactive to the mAb. By truncating the C protein, we identified a linear epitope recognized by the mAb, corresponding to amino acids 61TQDGLYHNKN70 of the CSFV C protein, which is well conserved among pestiviruses. Laser confocal analysis showed that the C protein mainly locates in the cellular nucleoplasm and nucleolus of PK-15 cells. The results have implications for further study of CSFV replication.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virus Research - Volume 156, Issues 1–2, March 2011, Pages 134-140
نویسندگان
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