کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6154376 1597727 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Potential of curcumin-mediated photodynamic inactivation to reduce oral colonization
ترجمه فارسی عنوان
پتانسیل غیرفعال سازی فوتودینامیک توسط کورکومین برای کاهش کلونیزاسیون دهان
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
چکیده انگلیسی


- A water-soluble mixture of curcuminoids (CRM) was proposed as photosensitizer.
- CRM-mediated PDI significantly reduced the amount of pathogens in human saliva.
- PDI showed similar results to 1-min CLX.

ObjectiveThe present study assessed the susceptibility of salivary pathogens to photodynamic inactivation (PDI), mediated by a water-soluble mixture of curcuminoids (CRM) and LED light.MethodsA 10 mL sample of unstimulated saliva was collected from volunteers. The inoculum was prepared using 9 mL of saline and 1 mL of saliva. Inoculum suspensions were divided into 14 groups and treated according to the description below. Groups that received the PDI treatment (light for 1 min or 5 min and 1.5 g/L or 3.0 g/L of CRM concentration) were called C1.5L1.8, C1.5L9.0, C3.0L1.8, C3.0L9.0. To evaluate the CRM decontamination alone, the C1.5/1,C1.5/5,C3.0/1 and C3.0/5 groups were assessed. Likewise, light alone was evaluated through the L1.8 and L9.0 groups. Chlorhexidine at 0.12% (CLX) for 1 or 5 min was used for the positive control groups (CLX1 and CLX5, respectively); saline was used for 1 or 5 min (CTR1, CTR5, respectively) for the negative control groups. After the tests, serial dilutions were performed, and the resulting samples were plated on blood agar in microaerophilic conditions. The number of colony forming units (CFU/mL) was determined and log10-transformed. Data were analyzed using a One-way Analysis of Variance with Welch correction, followed by the Games Howell's test (α = 0.05). Log reduction (LR) measure for antimicrobial efficacy was also calculated using data from the CTR5 as untreated samples.ResultsThe CHX5 showed the best antimicrobial result, followed by the CLX1. The antimicrobial effect of CRM was more pronounced when associated with light (PDI), but significantly lower than the CLX5 effect. The C3.0L9.0 protocol showed similar results to the CLX1.ConclusionThe results show that PDI with CRM at the studied concentrations is as effective for oral decontamination in clinical dental care conditions as the CLX at 0.12% for 1 min.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Photodiagnosis and Photodynamic Therapy - Volume 15, September 2016, Pages 46-52
نویسندگان
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