کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6263796 | 1613917 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhancement of FGF-1 release along with cytosolic proteins from rat astrocytes by hydrogen peroxide
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کلمات کلیدی
TLCDMEMFCSRT-PCRHDLFGF-1DPBSTCABSA - BSADulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoAstrocytes - آستروسیتbovine serum albumin - آلبومین سرم گاوapo - آپوapolipoprotein - آپولیپوپروتئینtrichloroacetic acid - اسید ترشکلراکتیکSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدOxidative stress - تنش اکسیداتیوCNS - دستگاه عصبی مرکزیblood brain barrier - سد خونی مغزیBBB - سد خونی مغزیfetal calf serum - سرم گوساله جنینcentral nervous system - سیستم عصبی مرکزیFibroblast growth factor 1 - عامل رشد فیبروبلاست 1Long-term culture - فرهنگ بلندمدتDulbecco's phosphate-buffered saline - فسفات باسیل نمک Dulbeccolactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH high density lipoproteins - لیپوپروتئینهای چگالی بالاCerebrospinal fluid - مایع مغزی نخاعیCSF - مایع مغزی نخاعیreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسTLC, Thin layer chromatography - کروماتوگرافی لایه نازک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The treatment of rat astrocytes with 100 µM hydrogen peroxide (H2O2) for 10 min enhanced FGF-1 release without inducing apoptosis. The conditioned medium prepared from the cells cultured in a fresh medium after the treatment with H2O2 had the FGF-1-like activities, which enhanced cholesterol synthesis, signalings to phosphorylate Akt and ERK, and apoE secretion. The oxidative stress induced by H2O2 enhanced the release of cytosolic proteins such as HSP70 and HSP90 in addition to FGF-1. Antioxidants such as ascorbic acid and ebselen suppressed the release of cytosolic proteins induced by H2O2 treatment. The addition of lipoproteins such as low density lipoproteins (LDL), furthermore, canceled H2O2-induced release of FGF-1 and cytosolic proteins. Proteolysis of cytosolic proteins in the H2O2-treated rat astrocytes was enhanced in the presence of exogenous trypsin, which was attenuated by the pretreatment with LDL, suggesting that H2O2 increases the permeability of the membrane of cells, which was prevented by the addition of lipoproteins. These findings suggest that oxidative stress is one of the candidates which triggers FGF-1 release from astrocytes in the brain, and that the lipid homeostasis in the cell membrane may regulate H2O2-induced release of FGF-1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1522, 19 July 2013, Pages 12-21
Journal: Brain Research - Volume 1522, 19 July 2013, Pages 12-21
نویسندگان
Jin-ichi Ito, Yuko Nagayasu, Mariko Hoshikawa, Koichi H. kato, Yutaka Miura, Kiyofumi Asai, Hideki Hayashi, Shinji Yokoyama, Makoto Michikawa,