کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6272461 | 1614781 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of ampicillin, cefazolin and cefoperazone treatments on GLT-1 expressions in the mesocorticolimbic system and ethanol intake in alcohol-preferring rats
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کلمات کلیدی
NACphosphorylated-AKTCefoperazoneALDH2CPZEAAT2GLT-1HRPGLMPFCAMPp-Akt - P-AKTAldehyde dehydrogenase-2 - آلدهید دهیدروژناز 2Ampicillin - آمپی سیلینEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدalcohol-preferring - الکل ترجیح می دهدanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceCefazolin - سفازولینprefrontal cortex - قشر prefrontalCerebrospinal fluid - مایع مغزی نخاعیCSF - مایع مغزی نخاعیGLM, General Linear Model - مدل خطی عمومیAlcohol intake - مصرف الکلNADH - نادانNAD, nicotinamide adenine dinucleotide - نیکوتینامید آدنین دینوکلئوتیدNucleus accumbens - هسته accumbensHorseradish peroxidase - پراکسیداز هوررادیشHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراglutamate - گلوتاماتglutamate transporter 1 - گلوتامات 1
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of ampicillin, cefazolin and cefoperazone treatments on GLT-1 expressions in the mesocorticolimbic system and ethanol intake in alcohol-preferring rats Effects of ampicillin, cefazolin and cefoperazone treatments on GLT-1 expressions in the mesocorticolimbic system and ethanol intake in alcohol-preferring rats](/preview/png/6272461.png)
چکیده انگلیسی
Chronic ethanol consumption is known to downregulate expression of the major glutamate transporter 1 (GLT-1), which increases extracellular glutamate concentrations in subregions of the mesocorticolimbic reward pathway. While β-lactam antibiotics were initially identified as potent upregulators of GLT-1 expression, only ceftriaxone has been extensively studied in various drug addiction models. Therefore, in this study, adult male alcohol-preferring (P) rats exposed chronically to ethanol were treated with other β-lactam antibiotics, ampicillin, cefazolin or cefoperazone (100 mg/kg) once daily for five consecutive days to assess their effects on ethanol consumption. The results demonstrated that each compound significantly reduced ethanol intake compared to the saline-treated control group. Importantly, each compound significantly upregulated both GLT-1 and pAKT expressions in the nucleus accumbens and prefrontal cortex compared to saline-treated control group. In addition, only cefoperazone significantly inhibited hepatic aldehyde dehydrogenase-2 enzyme activity. Moreover, these β-lactams exerted only a transient effect on sucrose drinking, suggesting specificity for chronically inhibiting ethanol reward in adult male P rats. Cerebrospinal fluid concentrations of ampicillin, cefazolin or cefoperazone have been confirmed using high-performance liquid chromatography. These findings demonstrate that multiple β-lactam antibiotics demonstrate efficacy in reducing alcohol consumption and appear to be potential therapeutic compounds for treating alcohol abuse and/or dependence. In addition, these results suggest that pAKT may be an important player in this effect, possibly through increased transcription of GLT-1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 295, 4 June 2015, Pages 164-174
Journal: Neuroscience - Volume 295, 4 June 2015, Pages 164-174
نویسندگان
P.S.S. Rao, S. Goodwani, R.L. Bell, Y. Wei, S.H.S. Boddu, Y. Sari,