کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273026 | 1614792 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
PKA is required for the modulation of spinal nociceptive information related to ephrinB-EphB signaling in mice
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کلمات کلیدی
NMDAPka catalytic subunitEphrinBEphBRTKTCIp-CREBPWLCCIN-methyl-d-aspartatePWTpKaCREBDRGdorsal root ganglion - گانگلیون ریشه پشتیcAMP - cAMPDMSO - DMSOCyclic adenosine monophosphate - آدنوزین مونوفسفات Cyclicpaw withdrawal threshold - آستانه برداشتن پاchronic constrictive injury - آسیب مغزی کمرنگpaw withdrawal latency - تاخیر در برداشت پنجهanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceRTK, Receptor tyrosine kinase - تیروزین کینازهای گیرنده ایCentral sensitization - حساس شدن مرکزیRoom temperature - دمای اتاقDimethyl sulfoxide - دیمتیل سولفواکسیدHyperalgesia - پردردی یا هایپرآلژزیcAMP-response element-binding protein - پروتئین متصل به عنصر cAMP-responseprotein kinase A - پروتئین کیناز A
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
EphB receptors and their ephrinB ligands are implicated in modulating of spinal nociceptive information processing. Here, we investigated whether protein kinase A (PKA), acts as a downstream effector, participates in the modulation spinal nociceptive information related to ephrinB-EphB signaling. Intrathecal injection of ephrinB2-Fc caused thermal hyperalgesia and mechanical allodynia, which were accompanied by increased expression of spinal PKA catalytic subunit (PKAca) and phosphorylated cAMP-response element-binding protein (p-CREB). Pre-treatment with H89, a PKA inhibitor, prevented the activation of CREB by ephrinB2-Fc. Inhibition of spinal PKA signaling prevented and reversed pain behaviors induced by the intrathecal injection of ephrinB2-Fc. Furthermore, blockade of the EphB receptors by intrathecal injection of EphB2-Fc reduced formalin-induced inflammatory, chronic constrictive injury (CCI)-induced neuropathic, and tibia bone cavity tumor cell implantation (TCI)-induced bone cancer pain behaviors, which were accompanied by decreased expression of spinal PKAca and p-CREB. Overall, these results confirmed the important involvement of PKA in the modulation of spinal nociceptive information related to ephrinBs-EphBs signaling. This finding may have important implications for exploring the roles and mechanisms of ephrinB-EphB signaling in physiologic and pathologic pain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 284, 22 January 2015, Pages 546-554
Journal: Neuroscience - Volume 284, 22 January 2015, Pages 546-554
نویسندگان
X.-L. Zhou, Y. Wang, C.-J. Zhang, L.-N. Yu, J.-L. Cao, M. Yan,