کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273494 | 1614801 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced excitatory synaptic network activity following transient group I metabotropic glutamate activation
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کلمات کلیدی
IPSCsmPSCsd,l-2-amino-5-phosphonovaleric acidACPD(1S,3R)-1-Aminocyclopentane-1,3-dicarboxylic acidmGluRsIPSCEPSCsCA3APVDHPG4-APTTX(S)-3,5-dihydroxyphenylglycine - (S) -3،5-دی هیدروکسی فنیل گلیسین4-aminopyridine - 4-آمینوپیریدینEPSC - EPSCoREpilepsy - بیماری صرعtetrodotoxin - تترو دوتوکسین excitatory postsynaptic currents - جریان های پسینپتیک هیجان انگیزinhibitory postsynaptic currents - جریانهای پسینپتیک مهارکنندهCNQX - سیانکیوایکسAHP - فرایند تحلیل سلسلهمراتبیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیmap - نقشهHippocampus - هیپوکامپ mitogen-activated protein - پروتئین فعال mitogenMetabotropic glutamate receptors - گیرنده های متابوتروپیک گلوتامات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Prolonged activation of group I metabotropic glutamate receptors (mGluRs) using the agonist (S)-3,5-dihydroxyphenylglycine (DHPG) produces long-lasting changes in the CA3 region of the hippocampal slice. Changes in CA3 pyramidal neuron excitability that follow DHPG exposure result in abnormal network activity manifest by epileptiform activity that consists of interictal and longer lasting ictal epileptiform discharges. In this study we evaluated changes in synaptic activity of CA3 neurons in rat hippocampal slices that occurred after exposure to DHPG. Whole-cell voltage-clamp recordings were made from visually identified CA3 neurons in control artificial cerebrospinal fluid at times greater than 1Â h after DHPG exposure. Compared to control slices, neurons from slices exposed to DHPG showed enhanced amplitude and frequency of spontaneously occurring excitatory postsynaptic currents (EPSCs) without a concurrent change in inhibitory postsynaptic current (IPSC) amplitude or frequency. Miniature EPSCs were not affected by DHPG exposure but mIPSCs occurred less frequently and were of reduced amplitude. IPSCs recorded in the presence of ionotropic glutamate receptor blockade occurred less frequently in neurons that had been exposed to DHPG. Monosynaptic-evoked IPSPs were also reduced in amplitude in neurons that had been exposed to DHPG. Taken together, these findings demonstrated an enhanced network excitability of the CA3 region and failure of compensatory synaptic inhibition. We propose that prolonged activation of group I mGluR that may occur under conditions of pathological glutamate release results in long-lasting changes in CA3 synaptic network activity and epileptiform activity driven by excessive synaptic excitation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 275, 5 September 2014, Pages 22-32
Journal: Neuroscience - Volume 275, 5 September 2014, Pages 22-32
نویسندگان
Y.-Z. Pan, P.A. Rutecki,