کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6273740 | 1614798 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Distribution of orexin-1 receptor-green fluorescent protein- (OX1-GFP) expressing neurons in the mouse brain stem and pons: Co-localization with tyrosine hydroxylase and neuronal nitric oxide synthase
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کلمات کلیدی
GFPOX1LPGiMVENOS1AMBLDTgOX2NTSDRNmRNA10N - 10 نانBAC - LACarea postrema - طبقه آخرlocus coeruleus - لوکوس سیرولئوسlateral paragigantocellular nucleus - هسته paragigantocellular جانبیnucleus ambiguus - هسته ابهامmedial vestibular nucleus - هسته داخلی وادیnucleus of the solitary tract - هسته دستگاه انفرادیdorsal raphe nucleus - هسته رافهdorsal motor nucleus of the vagus - هسته موتور پشتی وحشیlaterodorsal tegmental nucleus - هسته چرکین زاgreen fluorescent protein - پروتئین فلورسنت سبزbacterial artificial chromosome - کروموزوم مصنوعی باکتریایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We used a reporter mouse line in which green fluorescent protein (GFP) was inserted into the orexin-1 receptor (OX1) locus to systematically map the neuroanatomical distribution of the OX1 receptor in the mouse brainstem and pons. Here, we show that the OX1 receptor is expressed in a select subset of medullary and pontine nuclei. In the medulla, we observed OX1-GFP expression in the cuneate, gracile, dorsal motor nucleus of the vagus (10N), nucleus of the solitary tract and medullary raphe areas. In the pons, the greatest expression was found in the locus coeruleus (LC) and dorsal raphe nucleus (DRN). High to moderate expression was found in the pedunculopontine tegmental nucleus (PPTg), laterodorsal tegmental nucleus, A5 noradrenergic cell group (A5) and the periaqueductal gray. Double-labeling with neuronal nitric oxide synthase (NOS1) revealed extensive co-localization in cell bodies and fibers of the 10N, A5 cell group and the PPTg. Double-staining with tyrosine hydroxylase revealed extensive co-expression in the LC, DRN and the lateral paragigantocellularis cell group in the ventral medulla. Our findings faithfully recapitulate the findings of OX1 mRNA expression previously reported. This is the first study to systematically map the neuroanatomical distribution of OX1 receptors within the mouse hindbrain and suggest that this OX1-GFP transgenic reporter mouse line might be a useful tool with which to study the neuroanatomy and physiology of OX1 receptor-expressing cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 278, 10 October 2014, Pages 253-264
Journal: Neuroscience - Volume 278, 10 October 2014, Pages 253-264
نویسندگان
A. Darwinkel, D. StaniÄ, L.C. Booth, C.N. May, A.J. Lawrence, S.T. Yao,