کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6274390 | 1614822 | 2013 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Opiate exposure and withdrawal dynamically regulate mRNA expression in the serotonergic dorsal raphe nucleus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
Tryptophan hydroxylase2Tyrosine kinase receptor BTrkBDRNVTAtph2CRFmRNA5-HTCPPMORBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز μ-Opioid receptor - μ-گیرنده اپیوئیدStress - استرس یا فشار روانیGene expression - بیان ژنSERT - سختSerotonin - سروتونینserotonin transporter - سروتونین حمل کنندهconditioned place-preference - شرایط ترجیحی شرایطforced swim - شنا کردن اجباریBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزmorphine - مورفینventral tegmental area - ناحیه تگمنتوم شکمیdorsal raphe nucleus - هسته رافهpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازcorticotrophin-releasing factor - کورتیکوتروپین آزاد کننده فاکتور
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Previous results from our lab suggest that hypofunctioning of the serotonergic (5-HT) dorsal raphe nucleus (DRN) is involved in stress-induced opiate reinstatement. To further investigate the effects of morphine dependence and withdrawal on the 5-HT DRN system, we measured gene expression at the level of mRNA in the DRN during a model of morphine dependence, withdrawal and post withdrawal stress exposure in rats. Morphine pellets were implanted for 72 h and then either removed or animals were injected with naloxone to produce spontaneous or precipitated withdrawal, respectively. Animals exposed to these conditions exhibited withdrawal symptoms including weight loss, wet dog shakes and jumping behavior. Gene expression for brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor B (TrkB), corticotrophin releasing-factor (CRF)-R1, CRF-R2, alpha 1 subunit of the GABAA receptor (GABAA-α1), μ-opioid receptor (MOR), 5-HT1A receptor, tryptophan hydroxylase2 (TPH2) and the 5-HT transporter was then measured using quantitative real-time polymerase chain reaction at multiple time-points across the model of morphine exposure, withdrawal and post withdrawal stress. Expression levels of BDNF, TrkB and CRF-R1 mRNA were decreased during both morphine exposure and following 7 days of withdrawal. CRF-R2 mRNA expression was elevated after 7 days of withdrawal. 5-HT1A receptor mRNA expression was decreased following 3 h of morphine exposure, while TPH2 mRNA expression was decreased after 7 days of withdrawal with swim stress. There were no changes in the expression of GABAA-α1, MOR or 5-HT transporter mRNA. Collectively these results suggest that alterations in neurotrophin support, CRF-dependent stress signaling, 5-HT synthesis and release may underlie 5-HT DRN hypofunction that can potentially lead to stress-induced opiate relapse.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 254, 19 December 2013, Pages 160-172
Journal: Neuroscience - Volume 254, 19 December 2013, Pages 160-172
نویسندگان
J.W. Lunden, L.G. Kirby,