کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6278155 | 1295796 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rhesus monkey tryptophan hydroxylase-2 coding region haplotypes affect mRNA stability
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کلمات کلیدی
selective 5-HT reuptake inhibitorCystatin AcorneodesmosinCdsn5-HTMDR1OPRM1DRD2FBSTphDRBtph2amino acid - آمینو اسیدattention deficit hyperactivity disorder - اختلال کمتوجّهی _ بیشفعالیGene expression - بیان ژنADHD - بیشفعالیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاfetal bovine serum - سرم جنین گاوSSRI - مهارکنندههای بازجذب سروتونینtryptophan hydroxylase - هیدروکسیلاز تریپتوفانNonhuman primate - پریمات غیر انسانیSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتیدdopamine receptor D2 - گیرنده Dopamine D2Mu opioid receptor - گیرنده اپیدمی م
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rhesus monkey tryptophan hydroxylase-2 coding region haplotypes affect mRNA stability Rhesus monkey tryptophan hydroxylase-2 coding region haplotypes affect mRNA stability](/preview/png/6278155.png)
چکیده انگلیسی
Tryptophan hydroxylase-2 (TPH2) synthesizes neuronal 5-HT and its genetic variance is associated with numerous behavioral traits and psychiatric disorders. This study characterized the functional significance of two nonsynonymous single nucleotide polymorphisms (SNPs) (C74A and G223A) in rhesus monkey TPH2 (mTPH2). Four haplotypes of mTPH2 were cloned into pcDNA3.1 and stably transfected into PC12 cells. The levels of mTPH2 mRNA and protein were assessed by quantitative real-time PCR and Western blot, respectively, while the intracellular 5-HT was measured by enzyme-linked immunosorbent assay (ELISA). The variant A-A haplotype showed significantly higher levels of mTPH2 mRNA and protein, as well as significantly higher 5-HT production than the wild-type C-G haplotype, while the other two variant haplotypes (C-A and A-G) also tended to produce more 5-HT than C-G haplotype when stably expressed in PC12 cells. Both C74A and G223A were predicted to change mRNA secondary structure, and analysis of the mRNA stability showed that the wild-type C-G haplotype mRNA degrades more quickly than mRNAs of the mutant mTPH2 haplotypes in both stable PC12 and transient HEK-293 cells. This study demonstrates that nonsynonymous SNPs in mTPH2 can affect mRNA stability. Our findings provide an additional mechanism by which nonsynonymous SNPs affect TPH2 function, and further our understanding of TPH2 gene expression regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 155, Issue 2, 13 August 2008, Pages 485-491
Journal: Neuroscience - Volume 155, Issue 2, 13 August 2008, Pages 485-491
نویسندگان
G.-L. Chen, G.M. Miller,