کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6281400 | 1615115 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Resveratrol-induced SIRT1 activation promotes neuronal differentiation of human bone marrow mesenchymal stem cells
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کلمات کلیدی
Sirt1sirtuin1RSVPDEBM-MSCsBHAMSCsqPCRAMPKFBSPBScyclic AMP - AMP cyclicAMP-activated protein kinase - AMP-پروتئین کیناز فعال شده استcAMP - cAMPDMSO - DMSOMTT - MTTstandard deviation - انحراف معیارNeuronal differentiation - تمایز نورونRoom temperature - دمای اتاقDimethyl sulfoxide - دیمتیل سولفواکسیدResveratrol - رسوراترولfetal bovine serum - سرم جنین گاوMesenchymal stromal cells - سلولهای استرومایی مزانشیمی یا سلول های بنیادی مزانشیمیPhosphodiesterase - فسفو دی استرازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریbutylated hydroxyanisole - هیدروکسی آنیزول باتلاقیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Resveratrol-3,4â²,5-trihydroxy-trans-stillbene (resveratrol; RSV), a natural non-flavonoid polyphenol compound, provides protection against stress injury, excessive sunlight, ultraviolet radiation, infections, and invading fungi. There is increasing evidence that resveratrol, a sirtuin1 activator, plays a pivotal role in neuroprotection and neuronal differentiation. In this study, we investigated whether resveratrol induces neuronal differentiation of human bone marrow-mesenchymal stem cells (hBM-MSCs). Quantitative PCR results showed that resveratrol-treated MSCs (RSV-MSCs) had significantly increased expression of the neuroprogenitor markers Nestin, Musashi, CD133, and GFAP. When RSV-MSCs were differentiated with neuronal induction media (RSV-dMSCs), they exhibited a cell body and dendritic morphology similar to neurons. The number and neurite length of these RSV-dMSCs were significantly increased compared to differentiated MSCs (dMSCs). The RSV-dMSCs and dMSCs had significantly increased expression of the neuronal-specific marker genes Nestin, Musashi, CD133, GFAP, NF-M, MAP-2, and KCNH1. The RSV-dMSCs also showed a higher expression of the neuronal marker proteins, Nestin and NF-M, based on immunocytochemical staining and immunoblot analysis. This effect was abolished by the treatment of sirtuin1 inhibitor EX527. Therefore, we have shown that resveratrol treatment, along with the use of neuronal induction media, effectively stimulates neuronal cell differentiation of hBM-MSCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 584, 1 January 2015, Pages 97-102
Journal: Neuroscience Letters - Volume 584, 1 January 2015, Pages 97-102
نویسندگان
I-Seul Joe, Sin-Gu Jeong, Goang-Won Cho,