کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6288197 1615929 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bacterial and H2O2 stress-induced apoptosis-like events in Cryptococcus neoformans
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Bacterial and H2O2 stress-induced apoptosis-like events in Cryptococcus neoformans
چکیده انگلیسی
Cell death induced by adherence of Staphylococcus aureus was observed in Cryptococcus neoformans. To investigate this death mechanism, we examined three factors: actin dynamics using rhodamine-phalloidin staining, accumulation of reactive oxygen species (ROS) via conversion of 2′,7′-dichlorofluorescein diacetate to 2′,7′-dichlorofluorescein, and DNA fragmentation via terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL). In co-culture, actin visibly clumped and the percentage of ROS- and TUNEL-positive cells increased. The same cellular events were observed in C. neoformans after addition of hydrogen peroxide (H2O2), creating a bacterial cell-free system. After H2O2 treatment and cell disruption, C. neoformans proteins in soluble fraction were separated by two-dimensional (2-D) electrophoresis. A voltage-dependent ion-selective channel was identified as one of the stress-enhanced proteins. Thus, the death of C. neoformans was accompanied by decreased actin turnover, enhanced ROS accumulation, and DNA fragmentation. Cell death appeared similar to apoptosis via mitochondrial pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 159, Issues 9–10, November–December 2008, Pages 628-634
نویسندگان
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