Trypanosoma brucei: Inhibition of acetyl-CoA carboxylase by haloxyfop

► Examined effects of acetyl-CoA carboxylase inhibitor haloxyfop on Trypanosoma brucei. ► Haloxyfop inhibited T. brucei acetyl-CoA carboxylase activity in vitro. ► Haloxyfop inhibited the growth of T. brucei in culture. ► Haloxyfop did not affect fatty acid elongation of T. brucei in culture. ► Haloxyfop likely has off-target effects on T. brucei.