کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6309523 | 1618875 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil](/preview/png/6309523.png)
چکیده انگلیسی
A strain CSB 9 isolated from chromite mine soil of Sukinda, India was identified as Bacillus amyloliquefaciens based on biochemical and 16S rRNA gene sequencing. The strain exhibited relatively high tolerance to Cr(VI) (⩽900 mg Lâ1) and fast reduction rate of 2.22 mg Cr(VI) Lâ1 hâ1, under optimized conditions of 100 mg Lâ1 Cr(VI), pH 7 and temperature 35 °C within 45 h. Mechanism of Cr(VI) reduction as well as nature and fate of the reduced product were studied to determine the scope of removal of reduced Cr(III) end product. AAS analyses of the culture products treated with Cr(VI) for 45 h showed the distribution of Cr(III) in pellet and culture supernatant to be 37.4 ± 1.7 and 62.6 ± 3.4 mg Lâ1, respectively. In SEM images, the bacterial pellets with Cr(VI) treatment appeared coagulated, rough and porous whereas the pellets without Cr(VI) treatment appeared regular, smooth and non-porous in structure. SEM-EDX of the bacterial precipitates under Cr(VI) treatment revealed immobilization of Cr(III) species on the bacterial cell surface. Further Raman spectroscopy analysis confirmed the presence of Cr(III) species, with characteristic peak at around 600 cmâ1. TEM-EDX study of the bacterial precipitates under Cr(VI) treatment showed intracellular deposition of Cr(III) which are in nanometric range. Further characterization of reduced product by XRD, FT-IR and SAED analyses suggested the formation of poorly crystalline end products. A Cr(VI) removal mechanism considering both the surface immobilization and intracellular accumulation of Cr(III) along with the formation of coagulated cell precipitate by living B. amyloliquefaciens was suggested.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 96, February 2014, Pages 112-121
Journal: Chemosphere - Volume 96, February 2014, Pages 112-121
نویسندگان
Sasmita Das, Jigni Mishra, Saroj Kumar Das, Sony Pandey, Danda Srinivas Rao, Anindita Chakraborty, Mathummal Sudarshan, Nigamananda Das, Hrudayanath Thatoi,