Integrated biomarker analysis in the earthworm Lumbricus terrestris: Application to the monitoring of soil heavy metal pollution

► A multi-biomarker response was validated in field in Lumbricus terrestris. ► Heavy metal exposure alters earthworm hemoglobin concentration in field. ► Granulocyte size changes were demonstrated as valuable exposure biomarker in field. ► Results provide a sensitive tool for metal pollution monitoring in the soil.