New insights into the characterization of the binding of tetracycline analogues with lysozyme: A biophysical study

► TCs can interact with lysozyme with one binding site to form complex. ► Electrostatic forces play a major role in the formation of complex. ► TCs interact with the key active-site residues Glu 35 or Asp 52 in lysozyme cleft. ► Lysozyme's secondary structure and microenvironment were altered for bound TCs.