کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6367562 | 1316843 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of antimicrobial resistant bacteria in rivers: Insights into the cultivation bias
ترجمه فارسی عنوان
شناسایی باکتری های مقاوم به ضد میکروبی در رودخانه ها: نگاهی به تعصب کشت
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کلمات کلیدی
فاضلاب آلوده رودخانه، ضد میکروبی، مقاومت ضد میکروبی، ترکیب جامعه باکتریایی،
موضوعات مرتبط
مهندسی و علوم پایه
علوم زمین و سیارات
فرآیندهای سطح زمین
چکیده انگلیسی
In the present study, the antimicrobial resistant (AR) bacteria were quantified and identified in different river samples using in parallel a culture-based approach and a culture-independent one. The objective was to evaluate the importance of the cultivation bias when studying antimicrobial resistance among environmental bacteria. Three different river samples covering a gradient of anthropic influence were tested and three different antimicrobial compounds were used as selective agents: amoxicillin, tetracycline and sulfamethoxazole. From a quantitative point of view, our results highlight the importance of the culture media used, as for the same sample and the same selective agent significant differences were observed in the counts of culturable AR bacteria depending on the culture media used. The identification of AR bacteria through culture or culture-independent methods put on evidence AR bacterial communities that differ dramatically: γ-proteobacteria and more specifically Aeromonadaceae dominated among the isolates while β-proteobacteria (Comamonadaceae), dominated among the sequences obtained without culture. Altogether these results highlight the necessity to develop a methodological consensus preferably without culture, to approach this important topic in the coming years.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Water Research - Volume 47, Issue 14, 15 September 2013, Pages 4938-4947
Journal: Water Research - Volume 47, Issue 14, 15 September 2013, Pages 4938-4947
نویسندگان
Tamara Garcia-Armisen, Adriana Anzil, Pierre Cornelis, Marc Chevreuil, Pierre Servais,