کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6391621 | 1628417 | 2014 | 5 صفحه PDF | دانلود رایگان |
- Rapid and sensitive capture of LM was achieved using novel IMBs method.
- IMS with streptavidin-biotin system for capturing LM was systematically studied.
- Provide the appropriate selection of IMBs for developing isolation of pathogens.
- The IMBs method can be used to effectively capture LM in food matrix.
Immunomagnetic separation was used to decrease the assay time for Listeria monocytogenes (LM) isolation. The coupling conditions and immunomagnetic bead (IMB) capture process were optimized. Results showed that the incubation time and separation time for 0.1Â mg of 180Â nm immunomagnetic nanobeads (IMB-S-180) coupled with monoclonal antibody using a streptavidin-biotin system was 45 and 3Â min, respectively. Magnetic separation was affected by the separation medium and magnetic density of the magnetic separator. Capture efficiency (CE) was 94.12% when the magnetic density was 1.5Â T, using 1Â ml of PBS as separation medium. The IMBs exhibited highly specific binding with LM strains (CEÂ >Â 90%) but low binding with non-target bacteria (CEÂ <Â 5%). The CE of IMBs (0.1Â mg/ml) against LM in ground beef and milk samples were 25.1% and 91.2%, respectively. These results can serve as a guide for the appropriate selection of IMBs for developing isolation processes for pathogens.
Journal: Food Control - Volume 45, November 2014, Pages 138-142