کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6392091 1330436 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of bacillomycin D from Bacillus subtilis fmbJ and its inhibition effects against Aspergillus flavus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Identification of bacillomycin D from Bacillus subtilis fmbJ and its inhibition effects against Aspergillus flavus
چکیده انگلیسی
The study aimed to determine the structure and component of the antifungal substances against Aspergillus flavus produced by Bacillus subtilis fmbJ. With separation and purification, four of antifungal substances against A. flavus were obtained. By means of ESI-MS/CID analysis, their molecular weight was determined as being 1030D, 1044D, 1058D, 1072D, and the amino acid component of the antifungal substances were identified as being Asn-Tyr-Asn-Pro-Glu-Ser-Thr, which agreed with amino acid sequence of bacillomycin D. The mechanism of bacillomycin D against A. flavus was investigated. Bacillomycin D exhibited a high antifungal effect on the mycelium growth, sportulation and spore germination of A. flavus with the inhibition rate of 85.72%, 98.10% and 96.63% respectively under 200 μg/mL of purified bacillomycin D. It was found that bacillomycin D could injure the cell wall and cell membrane of the hypha and spore, then, cytoplasms and organelles inside the cell were exuded and form empty hole by observation of SEM (Scanning Electron Microscopy) and TEM (Transmission Electron Microscopy). The growth of A. flavus on corns could be completely inhibited in the presence of bacillomycin D at the concentration of 200 μg/g to 400 μg/g.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 36, Issue 1, February 2014, Pages 8-14
نویسندگان
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