کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6392164 | 1628425 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antimicrobial resistance determinants in antibiotic and biocide-resistant gram-negative bacteria from organic foods
ترجمه فارسی عنوان
عوامل تعیین کننده مقاومت ضد میکروبی در باکتری های گرم منفی مقاوم به آنتی بیوتیک و باکتری از غذاهای ارگانیک
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کلمات کلیدی
باکتری های گرم منفی، غذاهای ارگانیک، ژن های مقاومت ضد میکروبی، پمپ های خروجی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
In the present study, a collection of 29 Gram-negative bacteria selected from organic foods according to antibiotics and biocide resistance were screened for multidrug efflux pumps and specific antibiotic resistance genes. The acrB pump gene of the AcrAB-TolC system was detected in all isolates. EfrAB determinants efrA and efrB were frequently detected (Enterobacter, Pantoea, Salmonella), while other efflux pump genes (norE, mepA mdeA, norC) were detected only in some Enterobacter and Pantoea isolates. Genes encoding resistance to quaternary ammonium compounds qacE and qacJ were detected only in Enterobacter isolates, while sugE was detected in Enterobacter and in one Pantoea isolate. The aminoglycoside-resistance ant(4_)-Ia gene was detected in Enterobacter, Pantoea agglomerans and Klebsiella oxytoca. The antibiotic resistance determinant most frequently found was lsa gene (Enterobacter, Pantoea and Salmonella isolates). Erythromycin resistance genes (ereA, ereB) were detected among Enterobacter, Klebsiella or Salmonella. The macrolide efflux pump msrA and the phosphotransferase type I mphA genes were detected in Salmonella. Other resistance determinants analyzed (mdfA, yhiUV, evgA, 455emeA, yvcC, qacA/B, qacC, qacG, qacH, norA, norB, norC, sdrM, sepA, mecA, emrE, smr, bla, cat, aac(6_)-Ie-aph(2_)-Ia, aph(2_)-Ib, aph(2_)-Ic, aph(2_)-Id, aph(3_)-IIIa, ermA, ermB, ermC, and mefA genes) were not detected in any isolate. Results from the present study suggest that the main mechanisms of antibiotic and biocide resistance in Gram-negative bacteria from organic foods rely on different kinds of efflux pumps of broad substrate specificity. The indiscriminate use of biocides in the food chain should be revised in order to minimize persistence of reservoirs of antimicrobial resistance in the food chain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 37, March 2014, Pages 9-14
Journal: Food Control - Volume 37, March 2014, Pages 9-14
نویسندگان
Miguel Ángel Fernández Fuentes, Elena Ortega Morente, Hikmate Abriouel, Rubén Pérez Pulido, Antonio Gálvez,