کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6392422 1330438 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communicationDevelopment of triplex PCR for simultaneous detection of maize, wheat and soybean
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Short communicationDevelopment of triplex PCR for simultaneous detection of maize, wheat and soybean
چکیده انگلیسی


- New maize-specific and soybean-specific PCR primers were designed.
- The 101 bp PCR product of the lectin gene is a suitable soybean-specific marker.
- The 140 bp PCR product of the invertase gene is a proper maize-specific marker.
- The novel triplex PCR was developed for detection of maize, wheat and soybean.

A reliable and fast detection of important food plants, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and soybean (Glycine max L.) is of particular interest for food authenticity and safety assessment. In this study, the novel multiplex polymerase chain reaction (PCR) method was developed for the rapid qualitative detection of soybean, maize and wheat. To this purpose, new soybean-specific and maize-specific PCR primers were designed. Their specificity was assayed by uniplex PCRs with different plant species, namely maize, soybean, wheat, oats (Avena sativa), and barley (Hordeum vulgare L). Gel electrophoresis of the amplification products demonstrated high specificity of both primer pairs for identification of relevant species. Subsequently, based on the developed DNA markers, the species-specific triplex PCR targeting maize invertase gene, soybean lectin gene and wheat low-molecular-weight glutenin subunit was developed and optimized for simultaneous identification of these three plant species. The developed PCR method enables specific, effective and rapid detection of maize, wheat and soybean and may be used for food analysis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 34, Issue 2, December 2013, Pages 698-702
نویسندگان
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