کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6393505 1330453 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communicationMonitoring milk for antibodies against recombinant bovine somatotropin using a microsphere immunoassay-based biomarker approach
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Short communicationMonitoring milk for antibodies against recombinant bovine somatotropin using a microsphere immunoassay-based biomarker approach
چکیده انگلیسی

Recombinant bovine somatotropin (rbST) can be used to enhance milk production in dairy cattle. This is permitted in several countries but unauthorized in the European Union. Antibodies, which are produced endogenously in response to rbST administration, can be detected as a biomarker for indicating rbST (ab)use. For the first time, a fast and easy-to-perform screening assay for anti-rbST antibodies has been developed and applied to raw milk samples. This flow cytometric immunoassay (FCIA) is capable of discriminating between milk from rbST-treated animals and untreated animals. In accordance with literature, 67% of the rbST-treated animals responded positively with antibody production, whereas 94% of the untreated animals did not. The analysis of simulated tank milk samples showed more than 95% of the milk mixtures as truly positive for rbST treatment, indicating that the 33% physiologically non-responding cows will not be a problem when pooled tank milk samples are considered. FCIA biomarker responses in raw milk were specific for rbST and also obtained in pasteurized milk of rbST-treated animals. Using milk as a sample matrix for detection has the advantages of non-invasive sampling, and for tank milk analysis at the farm only one milk sample is needed to screen the whole farm for rbST (ab)use.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 26, Issue 1, July 2012, Pages 68-72
نویسندگان
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