Quantitative detection of viable foodborne E. coli O157:H7, Listeria monocytogenes and Salmonella in fresh-cut vegetables combining propidium monoazide and real-time PCR

► Real-time PCR (qPCR) assays cannot distinguish between DNA from dead and live cells. ► DNA binding molecules allow specific detection of viable cells. ► PMA and Reagent D were compared for the detection of foodborne pathogens. ► PMA performed better for the three pathogens. ► PMA-qPCR was able to specifically detect viable cells din pure cultures and food samples.