Development of reverse transcriptase polymerase chain reaction enzyme-linked immunosorbent assay for the detection of hepatitis A virus in vegetables

► RT-PCR-ELISA for HAV is sensitive and reliable detection technique. ► RT-PCR-ELISA for HAV was used to detect artificially contaminated HAV in vegetables. ► The sensitivity of RT-PCR-ELISA is 10-100 fold higher than RT-PCR.