Limonin 7-methoxime interferes with Escherichia coli biofilm formation and attachment in type 1 pili and antigen 43 dependent manner

► Determination of key structural features for anti-QS activity in V. harveyi. ► C-7 modification enhances the potency against E. coli biofilm. ► Limonin methoxime interfere with type-1-pili and Ag43 to inhibit E. coli attachment. ► Limonin methoxime significantly reduced the attachment of EHEC to spinach leaves.