Rapid detection of fish major allergen parvalbumin using superparamagnetic nanoparticle-based lateral flow immunoassay

► A competitive lateral flow immunoassay for parvalbumin (Pa) detection is developed. ► Superparamagnetic nanoparticle is introduced to realize quantitative detection. ► Assay shows wide linear range, high sensitivity and good validity. ► Detection time is less than 20 min and signal remained stable during storage.