Reduction in vitro of the minor Fusarium mycotoxin beauvericin employing different strains of probiotic bacteria

The interaction between the minor Fusarium mycotoxins BEA and 13 bacterial strains characteristic of the gastrointestinal tract as Bifidobacterium longum, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium adolescentes, Lactobacillus rhamnosus, Lactobacillus casei-casei, Lactobacillus plantarum, Eubacterium crispatus, Salmonella fecalis, Salmonella termofilus, Lactobacillus ruminis, Lactobacillus casei and Lactobacillus animalis was studied.The fermentations were carried out in the liquid medium of MRS during 4, 12, 16, 24 and 48 h at 37 °C, under anaerobic conditions.Levels of BEA in the fermentation liquid, on the cell walls and on the internal part of the cells were determined using liquid chromatography coupled to the mass spectrometry detector (LC-MS/MS). Results showed that the bacteria reduced the concentration of the BEA present in the medium, part of the mycotoxin was adsorbed by cell wall and part internalized by the bacteria. All the bacteria analyzed in this study showed a significant BEA reduction during the fermentation process, in particular the mean diminution resulted variable from 66 to the 83%.

► The reduction in vitro of BEA employing lactic acid bacteria. ► LC-MS determination of adducts between BEA and the bacterial cells. ► Identification of bacterial strains for industrial application.