کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6394517 1330470 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Applicability assessment of a real-time PCR assay for the specific detection of bovine, ovine and caprine material in feedstuffs
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Applicability assessment of a real-time PCR assay for the specific detection of bovine, ovine and caprine material in feedstuffs
چکیده انگلیسی

A TaqMan real-time polymerase chain reaction (PCR) method was developed for specific detection of bovine, ovine and caprine processed animal protein (PAP) in industrial feedstuffs. The method uses species-specific primers and probes targeting short mitochondrial D-loop sequences, and a positive amplification control based on 18S rRNA gene. The applicability of the real-time PCR protocol was assessed through analysis of 126 industrial feed samples that were manufactured to reproduce rendering processes of commercial feeds destined for farmed animals. The assay successfully classified samples as positive or negative according to the ruminant composition, enabling qualitative detection of banned material in feeds at levels as low as 0.1%. Although the method provides quantitative potential, results suggest that the real quantitative capability of the assay is limited by the existing variability in terms of composition and processing treatments of the feeds, which affect the amount and quality of amplifiable DNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 22, Issue 8, August 2011, Pages 1189-1196
نویسندگان
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