کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6451945 | 1416984 | 2017 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research paperMolecular mass of Poly-3-hydroxybutyrate (P3HB) produced by Azotobacter vinelandii is influenced by the polymer content in the inoculum Research paperMolecular mass of Poly-3-hydroxybutyrate (P3HB) produced by Azotobacter vinelandii is influenced by the polymer content in the inoculum](/preview/png/6451945.png)
- P3HB content in the inoculum determines the Mw of P3HB produced by A. vinelandii.
- Inoculum composition affects the P3HB productivity in A. vinelandii cultures.
- An inverse relationship between the final Mw of P3HB and productivity was found.
Poly-3-hydroxybutyrate (P3HB) is a biopolymer produced by Azotobacter vinelandii. The physicochemical properties and applications of P3HB are strongly influenced by its weight-average molecular mass (Mw), and in A. vinelandii, it could be influenced by the culture conditions. The aim of this study was to evaluate the effect of the P3HB content of the inoculum on the Mw of the polymer produced by A. vinelandii OP in bioreactor cultures. A. vinelandii cells containing 20, 50 and 70% of P3HB were used as inoculum. The P3HB content in the inoculum affected the volumetric P3HB productivity (qP3HB) and the Mw of P3HB. Those cultures inoculated with cells containing 20% of P3HB, achieved the highest qP3HB (0.17 ± 0.018 gP3HB Lâ1 hâ1); whereas a P3HB content of 70% was reflected as a low qP3HB (0.021 ± 0.002 gP3HB Lâ1 hâ1). On the other hand, using an inoculum with 70% of polymer content, the Mw of the biopolymer remained stable at values close to 3200 kDa; whereas, when an inoculum with 20% of P3HB was used, the Mw decreased drastically during early stages of cultivation. These results show that manipulating the P3HB content of the inoculum is possible to produce biopolymers with a suitable Mw.
Journal: Journal of Biotechnology - Volume 259, 10 October 2017, Pages 50-55