کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6451986 1416984 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Placenta-specific gene manipulation in rabbits
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Placenta-specific gene manipulation in rabbits
چکیده انگلیسی


- Placenta-specific Gene Manipulation in Rabbits with lentiviral vectors.
- Microinjection of 8-16 cell stage embryos resulted extraembryonic gene expression.
- Strong but mosaic reporter gene expression in the placenta and yolk sac.

Lentiviral gene constructs can be efficiently and specifically delivered to trophoblast cell lineages in rodents. In vivo genetic manipulation of trophoblast cell lines enables functional and developmental studies in the placenta. In this report we show that genetic modification can be produced in the extraembryonic tissues of rabbits by lentiviral gene constructs. When 8-16 cell stage embryos were injected with lentiviral particles, strong reporter gene expression resulted in the rabbit placenta. The expression pattern displayed some mosaicism. A strikingly high degree of mosaic GFP expression was detected in some parts of the yolk sac, which is a hypoblast-derived tissue. Whereas expression of the reporter gene construct was detected in placentas and yolk sacs, fetuses never expressed the transgene. As rabbits are an ideal model for functional studies in the placenta, our method would open new possibilities in rabbit biotechnology and placentation studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 259, 10 October 2017, Pages 86-90
نویسندگان
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