Short Genome CommunicationsComplete genome sequence of Ruminococcaceae bacterium CPB6: A newly isolated culture for efficient n-caproic acid production from lactate

- The first complete genome sequence of a lactate-utilizing, caproate-producing Ruminococcaceae bacterium CPB6 is presented.
- Sets of genes involved in the fatty acid biosynthesis via acyl carrier protein (ACP) and coenzyme A (CoA) pathways were identified in the genome.
- Strain CPB6 is a powerful biocatalyst source for the bioproduction of CA from lactate-containing waste streams.

n-caproic acid (CA) is a valuable chemical feedstock for various industrial applications. Biological production of CA from renewable carbon sources has attracted a lot of attentions recently. We lately reported the new culture Ruminococcaceae bacterium CPB6, which was isolated from a microbiome for efficient CA production from lactate. To further elucidate its metabolism, we sequenced the whole genome of the strain. The size of the complete genome is 2,069,994 bp with 50.58% GC content; no plasmid was identified. Sets of genes involved in the fatty acid biosynthesis via acyl carrier protein (ACP) and coenzyme A (CoA) as well as lactate oxidation/reduction pathways were identified in the genome. These genes were inferred to be correlated with the CA production. The complete genome sequence provides essential information for the elucidation of the metabolism for CA production from lactate, and further improvement of the strain through genetic engineering for enhanced CA production and other biotechnological purposes.