ReviewSynergism of proteomics and mRNA sequencing for enzyme discovery

- Proteomic screening is employed for enzyme discovery for biotechnology industry.
- Targeted protein databases improve protein identification in proteomic screenings.
- Database generation by transcriptomics has been successfully employed for this purpose.

Enzyme catalyzed processes are increasingly complementing chemical manufacturing as new enzymes are being discovered. Although, many industrially applied biocatalysts have been identified by functional screenings technological advances in the omics fields have created a different path to access novelty. Here we describe how omics technologies, especially proteomics and transcriptomics, can complement each other in the aim of finding new enzymatic functions. Special emphasis is laid on how mRNA sequencing Zcan improve proteomic experiments by allowing the generation of high quality protein sequence databases, which subsequently facilitates protein identification.