کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6462922 | 1422151 | 2017 | 7 صفحه PDF | دانلود رایگان |
- A new 23-plex STR system contained 21 non-CODIS STR loci was validated.
- Applicable for 10 ul even 5 ul reaction volumes.
- Owned wide range of annealing temperatures with 58 °C to 62 °C.
Microreader⢠23sp ID system is a new 23-plex STR genotyping system that amplified 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290 and D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus in one reaction. Microreader⢠23sp ID system was validated according to the guidelines of “Validation Guidelines for DNA Analysis Methods (2012)” described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. Our results suggested that Microreader⢠23sp ID system is a useful tool for identification and parentage testing.
Journal: Forensic Science International: Genetics - Volume 27, March 2017, Pages 67-73