کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6463143 | 1361928 | 2016 | 10 صفحه PDF | دانلود رایگان |
SummaryOral lichen planus (OLP) is a complex immunological disorder, mediated in part by the release of cytokines from activated T-cells. Of late, two closely related T-helper (Th) cell subsets; regulatory T-cells (Tregs; FoxP3+) and Th17 cells (IL17+) have been described in various chronic inflammatory diseases. The aim of this study was to determine the expression of FoxP3 and IL17 in OLP using immunohistochemistry (IHC) and quantitative real-time reverse transcriptase polymerase chain reaction (qPCR). For IHC, formalin fixed, paraffin embedded archival specimens, an OLP group (n = 10) and a non-specific inflammatory (NSI) control group (n = 9) were used. In addition, 12 fresh tissue samples were used to determine gene expression of FoxP3 and IL17. Significantly more FoxP3+ cells were present in OLP than in NSI. IL17+ cells were significantly more frequent in the control tissues than in OLP. The gene expression experiments revealed a significantly higher expression of FoxP3 in OLP when compared to the controls. IL17 gene expression was not different between the groups. Double labelling immunofluorescence indicated co-localisation of IL17 with tryptase+ mast cells. These findings suggest FoxP3+ Tregs have a more prominent role in the pathogenesis of OLP when compared to IL17+cells.
Journal: Pathology - Volume 48, Issue 6, October 2016, Pages 564-573