کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6469907 1424106 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimization of an amperometric biosensor array for simultaneous measurement of ethanol, formate, d- and l-lactate
ترجمه فارسی عنوان
بهینه سازی آرایه بیوسنسور آمپرئومتری برای اندازه گیری همزمان اتانول، فرمالیت، دی-و ال-لاکتات
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی

The immobilization of NAD+-dependent dehydrogenases, in combination with a diaphorase, enables the facile development of multiparametric sensing devices. In this work, an amperometric biosensor array for simultaneous determination of ethanol, formate, d- and l-lactate is presented. Enzyme immobilization on platinum thin-film electrodes was realized by chemical cross-linking with glutaraldehyde. The optimization of the sensor performance was investigated with regard to enzyme loading, glutaraldehyde concentration, pH, cofactor concentration and temperature. Under optimal working conditions (potassium phosphate buffer with pH 7.5, 2.5 mmol L-1 NAD+, 2.0 mmol L-1 ferricyanide, 25 °C and 0.4% glutaraldehyde) the linear working range and sensitivity of the four sensor elements was improved. Simultaneous and cross-talk free measurements of four different metabolic parameters were performed successfully. The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge. Thereby, a promising approach for on-site monitoring of fermentation processes is provided.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 251, 10 October 2017, Pages 256-262
نویسندگان
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