|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|6472864||1424138||2016||6 صفحه PDF||سفارش دهید||دانلود رایگان|
- Electrodes were modified by an electrogenerated Ru(II) complex which demonstrates photosensitive properties and intercalating properties towards the stem-loop base pairing domain of cocaine aptamers.
- Cocaine aptamers were immobilized as mono-and double-fragment which showed different behaviour towards photocurrent generation.
- The binding of aptamer could be followed by photelectrochemistry and modelized using a Langmuir-Freundlich isotherm.
- Using the double-fragment aptamer, a label-free photoelectrochemical aptasensor was designed, exhibiting a LOD of 10Â nmolÂ Lâ1 and linear range of 1 10â8-5 10â4Â molÂ Lâ1.
A photoelectrode was designed by electrodeposition of a pyrrole monomer modified with a polypyridyl Ru(II) complex bearing benzo[i]dipyrido-[3,2-a:2â².3â²-c]phenazine (dppn) ligand. Owing to the intercalating properties of these immobilized complexes towards DNA double helix, cocaine aptamer was immobilized on the modified electrodes thanks to its stem-loop configuration in order to design a photoelectrochemical cocaine aptasensor. Especially using a double-fragment aptamer strategy, the binding of cocaine and the formation of the aptamer/cocaine complex was successfully observed and modeled by a Langmuir-Freundlich isotherm, giving access to an apparent dissociation constant Kd of 3.8Â mmolÂ Lâ1. The photoelectrochemical aptasensor exhibits a LOD of 10Â nmolÂ Lâ1 and linear range of 1 10â8-5 10â4Â molÂ Lâ1.
Journal: Electrochimica Acta - Volume 219, 20 November 2016, Pages 82-87