کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6481906 1557305 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gold nanoparticles enhance 5-fluorouracil anticancer efficacy against colorectal cancer cells
ترجمه فارسی عنوان
نانوذرات طلا باعث افزایش اثربخشی ضد سرطان 5-فلوروئوراسیس به سلولهای سرطانی روده بزرگ می شود
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
چکیده انگلیسی

5-Fluorouracil (5-FU), an antimetabolite drug, is extensively used in the treatment solid tumors. However, its severe side effects limit its clinical benefits. To enhance 5-FU anticancer efficacy and reduce its side effects it was loaded onto gold nanoparticles (GNPs) using two thiol containing ligands, thioglycolic acid (TGA) and glutathione (GSH). The GNPs were prepared at different 5-FU/ligand molar ratios and evaluated using different techniques. Anticancer efficacy of 5-FU/GSH-GNPs was studied using flow cytometry in cancerous tissue obtained from patients having colorectal cancer. The GNPs were spherical in shape and had a size of ∼9-17 nm. Stability of the GNPs and drug release were studied as a function of salt concentration and solution pH. Maximum 5-FU loading was achieved at 5-FU/ligand molar ratio of 1:1 and 2:1 for TGA-GNPs and GSH-GNPs, respectively. GNPs coating with pluronic F127 improved their stability against salinity. 5-FU release from GNPs was slow and pH-dependent. 5-FU/GSH-GNPs induced apoptosis and stopped the cell cycle progression in colorectal cancer cells. They also had a 2-fold higher anticancer effect compared with free 5-FU. These results confirm the potential of GNPs to enhance 5-FU anticancer efficacy.

Gold nanoparticles (GNPs) functionalized with two bifunctional ligands, thioglycolic acid (TGA) and glutathione (GSH) were successfully used as delivery system for 5-FU. 5-FU/GSH-GNPs achieved about 1.5-2-fold enhancement in 5-FU cytotoxic effect against colorectal cancer cells compared with free 5-FU.69

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Pharmaceutics - Volume 513, Issues 1–2, 20 November 2016, Pages 648-658
نویسندگان
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